| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Richardson, Davia K (14)
|
MRYMID |
24.30 |
24.56 |
0.26 |
| 2 |
Arnall, Ines M (14)
|
MRWEST |
24.51 |
24.78 |
0.27 |
| 3 |
Arnall, Alba (14)
|
MRWEST |
24.84 |
24.95 |
0.11 |
| 4 |
Wu, Natalie (14)
|
MRLIAC |
24.47 |
25.04 |
0.57 |
| 5 |
So, Leni A (14)
|
MRTS |
25.16 |
25.41 |
0.25 |
| 6 |
Wright, Alison E (14)
|
MRYFD |
25.47 |
25.61 |
0.14 |
| 6 |
Aydin, Zoey L (14)
|
MRPAC |
25.71 |
25.61 |
-0.10 |
| 8 |
Fishbein, Lauren B (14)
|
MRAGUA |
26.09 |
25.73 |
-0.36 |
| 9 |
Lin, Madison C (14)
|
MRLIAC |
25.76 |
25.75 |
-0.01 |
| 10 |
Goncharenko, Nikol (14)
|
MRAGUA |
25.94 |
25.83 |
-0.11 |
| 11 |
O'Donnell, Katherine A (14)
|
MRBGNW |
25.49 |
25.92 |
0.43 |
| 12 |
Cole, Alexandra T (14)
|
MRTVSC |
26.01 |
25.96 |
-0.05 |
| 13 |
Kelsch, Morgan E (14)
|
MRLIAC |
25.57 |
25.97 |
0.40 |
| 14 |
Ehrhardt, Victoria A (14)
|
MRFA |
25.93 |
26.01 |
0.08 |
| 15 |
Brady, Meghan E (14)
|
MRTVSC |
25.25 |
26.17 |
0.92 |
| 16 |
Ramirez, Lily L (14)
|
MRYFD |
25.73 |
26.21 |
0.48 |
| 17 |
Gironda, Kate E (14)
|
MRTVSC |
25.89 |
26.24 |
0.35 |
| 18 |
Franks, Ellie (14)
|
MRLIAC |
25.81 |
26.29 |
0.48 |
| 19 |
Skerritt, Katherine C (14)
|
MRHVD |
25.77 |
26.32 |
0.55 |
| 20 |
Reddington, Shannon E (14)
|
MRLIE |
25.56 |
26.35 |
0.79 |
| 21 |
McDonald, Cara B (14)
|
MRLIAC |
25.93 |
26.37 |
0.44 |
| 22 |
Harkins, Morgan P (14)
|
MRLIE |
26.07 |
26.39 |
0.32 |
| 23 |
Karellas, Caroline M (14)
|
MRYFD |
26.08 |
26.54 |
0.46 |
| 24 |
Bak, Hanna M (14)
|
MRQNS |
26.05 |
26.61 |
0.56 |
| 25 |
Broening-Chai, Naila A (14)
|
MRAGUA |
26.09 |
26.76 |
0.67 |
| 26 |
Yan, Madelyn (14)
|
MRQNS |
26.07 |
27.16 |
1.09 |
| 27 |
Olsen, Liv I (14)
|
MRAGUA |
25.89 |
27.38 |
1.49 |
| 28 |
Kelly, Holly (14)
|
MRCOND |
33.80 |
34.71 |
0.91 |
|
Zakharova, Dasha (14)
|
MRTVSC |
25.77 |
NS |
- |
|
Lent, Madeline J (14)
|
MRAGUA |
24.71 |
NS |
- |